Composite

Part:BBa_K4614102:Design

Designed by: Yao-han Huang   Group: iGEM23_CAU-China   (2023-10-06)


T7-RBS-FlgH(SpyTag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Please insert the sequence of SpyTag into the middle of the flgH gene and add the linker peptide before and after it, referring to the sequence given in this project, otherwise it may not be located correctly.


Source

In this project, FlgH is derived from the genome of E. coli K12, and SpyTag and other components are synthesized by the company.

References